IMPROVING THE EFFICIENCY OF PRECISE GENOME EDITING WITH CRISPR/CAS9 TO GENERATE GOATS OVEREXPRESSING HUMAN BUTYRYLCHOLINESTERASE

Improving the Efficiency of Precise Genome Editing with CRISPR/Cas9 to Generate Goats Overexpressing Human Butyrylcholinesterase

The CRISPR/Cas9 system is widely used for genome editing in livestock production, although off-target effects can occur.It is the main method to produce genome-edited goats by somatic cell nuclear transfer (SCNT) of CRISPR/Cas9-mediated genome-edited primary goat fetal fibroblast cells (GFFs).Improving the double-strand break (DSB) remtavares.com e

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