Improving the Efficiency of Precise Genome Editing with CRISPR/Cas9 to Generate Goats Overexpressing Human Butyrylcholinesterase
The CRISPR/Cas9 system is widely used for genome editing in livestock production, although off-target effects can occur.It is the main method to produce genome-edited goats by somatic cell nuclear transfer (SCNT) of CRISPR/Cas9-mediated genome-edited primary goat fetal fibroblast cells (GFFs).Improving the double-strand break (DSB) remtavares.com e